Botu Red Cushion

(1):

1. Aspire 3 mL of RedCushion with new syringe and needles, and deposit it at the bottom of the centrifugation tube.
2. Dilute the ejaculate with BotuSemen in the proportion of 1:1 v/v
3. Incline the tube to 45º and slowly deposit the diluted semen on the wall with plastic Pasteur pipettes so that no mixing occurs between the semen and the RedCushion
4. Centrifuge at 1000 xg for 20 minutes (Fanem centrifuges: 1000 xg = 2,700 rpm)
5. With help of a pipette remove the sperm “ring” deposited on top of RedCushion
6. Resuspend the sperm with BotuSemen or BotuCrio, according to the procedure to be held.

1. Dilute the ejaculate with BotuSemen in the proportion of 1:1 v/v
2. Insert the diluted semen into a centrifuge tube
3. With help of new syringe and needles deposit 3 mL of RedCushion under the diluted semen
4. Centrifuge at 1000 xg for 20 minutes (Fanem centrifuges: 1000 xg = 2,700 rpm)
5. Remove the supernatant
6. Aspire the formed pellet over the RedCushion
7. Resuspend the sperm with BotuSemen or BotuCrio, according to the procedure to be held.